In Vitro Phosphate Binding Capacity of a Dietary Supplement for Dogs and Cats
DOI:
https://doi.org/10.29169/1927-5951.2022.12.08Keywords:
Dietary supplement, pet, phosphate binder, renal disorderAbstract
Hyperphosphatemia has been recognized as a serious and frequent complication in dogs and cats with Chronic Kidney Disease (CKD). The veterinary approach is mainly based on reducing phosphorus in the diet and with phosphate (P) binders. In this in vitro study we compared seven supplements with a different combination of ingredients for dogs and cats at pH 3 and 7 to estimate the P binding capacity overtime. Our results confirmed the best binding capacity of supplements at acid compared to basic condition. The P binding capacity of two products containing mainly calcium carbonate, calcium lactate-gluconate and chitosan was higher compared to the other tested at the same conditions.
References
Sparkes AH, Caney S, Chalhoub S, Elliott J, Finch N, Gajanayake I, et al. ISFM Consensus guidelines on the diagnosis and management of feline chronic kidney disease. J Feline Med Surg 2016; 18(3): 219-39. https://doi.org/10.1177/1098612X16631234
Bernachon N, Fournel S, Gatto H, Monginoux P, McGahie D. Effect of a product containing the dietary phosphate binders calcium and magnesium carbonate associated with other reno-protectant substances (Pronefra®) on blood parameters and mineral balance in adult cats. Intern J Appl Res Vet Med. 2014; 12.
Martello E, Perondi F, Bruni N, Bisanzio D, Meineri G, Lippi I. Chronic kidney disease and dietary supplementation: effects on inflammation and oxidative stress. Vet Sci 2021; 8(11). https://doi.org/10.3390/vetsci8110277
Biasibetti E, Martello E, Bigliati M, Biasato I, Cocca T, Bruni N, et al. A long term feed supplementation based on phosphate binders in feline chronic kidney disease. Veterinary Research Communications 2018; 42(2): 161-7. https://doi.org/10.1007/s11259-018-9719-z
Martello E, Perondi F, Capucchio MT, Biasato I, Biasibetti E, Cocca T, et al. Efficacy of a new dietary supplement in dogs with advanced chronic kidney disease. Peer J 2020; 8: e9663. https://doi.org/10.7717/peerj.9663
Emmett M. A comparison of clinically useful phosphorus binders for patients with chronic kidney failure. Kidney international Supplement 2004; (90): S25-32. https://doi.org/10.1111/j.1523-1755.2004.09005.x
Wagner E, Schwendenwein I, Zentek J. Effects of a dietary chitosan and calcium supplement on Ca and P metabolism in cats. Berliner und Munchener tierarztliche Wochenschrift 2004; 117(7-8): 310-5.
Fritz K, Taylor K, Parmar M. Calcium Carbonate. 2022 Aug 1. In: StatPearls [Internet]. Treasure Island (FL): Stat Pearls Publishing 2022. PMID: 32965974.
Yaguchi A, Akahane K, Tsuchioka K, Yonekubo S, Yamamoto S, Tamai Y, et al. A comparison between the combined effect of calcium carbonate with sucroferric oxyhydroxide and other phosphate binders: an in vitro and in vivo experimental study. BMC Nephrol 2019; 20(1): 465. https://doi.org/10.1186/s12882-019-1655-9
Segev G, Bandt C, Francey T, Cowgill LD. Aluminum toxicity following administration of aluminum-based phosphate binders in 2 dogs with renal failure. J Vet Intern Med 2008; 22(6): 1432-5. https://doi.org/10.1111/j.1939-1676.2008.0206.x
Sheikh MS, Maguire JA, Emmett M, Santa Ana CA, Nicar MJ, Schiller LR, et al. Reduction of dietary phosphorus absorption by phosphorus binders. A theoretical, in vitro, and in vivo study. J Clin Invest 1989; 83(1): 66-73. https://doi.org/10.1172/JCI113886
King EJ. The colorimetric determination of phosphorus. Biochem J 1932; 26(2): 292-7. https://doi.org/10.1042/bj0260292
Polzin DJ. Chronic kidney disease in small animals. Vet Clin North Am Small Anim Pract 2011; 41(1): 15-30. https://doi.org/10.1016/j.cvsm.2010.09.004
Peitsch T, Matthes M, Brandenburg V, Epple M. An in vitro crystallization setup to assess the efficiency of different phosphate binders in nephrology: quantitative analytical considerations. Analytical Methods 2010; 2(7): 901-11. https://doi.org/10.1039/b9ay00325h
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Natascia Bruni, Elisa Martello, Raffaella Adami, Giorgia Meineri, Francesca Perondi, Annalisa Costale, Elena Rosso, Ilaria Lippi

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
How to Cite
Similar Articles
- Leelayuwat, S. Laddawan, Y. Kanpetta, M. Benja, D. Wongpan, O. Tunkamnerdthai, J. Wattanathorn, S. Muchimapura , J. Yamauchi, Quercetin Enhances Endurance Capacity via Antioxidant Activity and Size of Muscle Fibre Type 1 , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 2 (2012)
- Swati Jain, Saroj K. Verma, Vijay K. Singh , Som Nath Singh, Effect of Short Term Sibutramine Supplementation on Appetite Suppression and Related Metabolic Responses , Journal of Pharmacy and Nutrition Sciences : Vol. 2 No. 2 (2012)
- Andi Imam Arundhana , Muhammad Syafruddin Nurdin , Veni Hadju , Ansariadi , Andi Zulkifli, The Effect of Moringa-Based Supplementation on Fetal Birth Weight in Jeneponto Regency , Journal of Pharmacy and Nutrition Sciences : Vol. 8 No. 3 (2018)
- Sara Tutunchi, Mehdi Koushki, Nasrin Amiri-Dashatan, Hadi Khodabandehloo, Hossein Hosseini, Godratollah Panahi, Javad Hashemi, Amir Karbalaee-Hasani, Ziba Majidi, Mostafa Rezaei-Tavirani, Effect of Soy Isoflavones Supplementation on Adiponectin Levels in Postmenopausal Women , Journal of Pharmacy and Nutrition Sciences : Vol. 11 (2021)
- Amalia Purnamasari Zainal, Ririen Hardani , Amelia Rumi, Nutritional Formulation and Analysis, and Organoleptic Tests of Functional Chocolate Biscuits “Morisoya” –Supplementation of Moringa Leaves (Moringa oleifera L.) AND Soybeans (Glycine max (L.) Merill) , Journal of Pharmacy and Nutrition Sciences : Vol. 9 No. 1 (2019)
- Michael A. Sandoval, Hugh D. Smyth, Jason T. McConville, Dissolution Profiles of Lanoxin Tablets in Media Supplemented with Soluble and Insoluble Forms of Fiber , Journal of Pharmacy and Nutrition Sciences : Vol. 1 No. 2 (2011)
- José M. Moreno, Irene Leets, Rafael J. Puche, Ana M. Salazar, Jham F. Papale, Gloria Alvarado , María N. García-Casal, Low Dose β Carotene Supplementation Diminishes Oxidative Stress in Type 2 Diabetics and Healthy Individuals , Journal of Pharmacy and Nutrition Sciences : Vol. 3 No. 3 (2013)
- C.S. Devaki , K.S. Premavalli, Evaluation of Supplementation of Bittergourd Fermented Beverage to Diabetic Subjects , Journal of Pharmacy and Nutrition Sciences : Vol. 4 No. 1 (2014)
- D.A. Areshidze, L.D. Timchenko, M.A. Kozlova, I.A. Syomin, I.V. Rzhepakovsky, S.I. Piskov , V.N. Vakulin, Influence of Biologically Active Substance “STEMB” on a Morphofunctional Condition of a Liver and Kidneys of Rats at an Experimental Hepatorenal Syndrome , Journal of Pharmacy and Nutrition Sciences : Vol. 5 No. 3 (2015)
- Adebowale Emmanuel Aladejana, Graeme Bradley, Anthony Jide Afolayan, Phytochemical Screening and In-Vitro Antioxidant Activities of Various Extracts of Helichrysum petiolare Hilliard & B.L. Burtt used for the Treatment of Diabetes Mellitus in the Eastern Cape Province of South Africa , Journal of Pharmacy and Nutrition Sciences : Vol. 10 No. 6 (2020)
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Natascia Bruni, Elisa Martello, Valeria Gabriele, Francesca Perondi, Acid Suppressant Activity of Feed Dietary Supplements for Dogs , Journal of Pharmacy and Nutrition Sciences : Vol. 13 (2023)